Guide to Surgical Instruments for Rodent Catheterization

13 Jan.,2025

 

Guide to Surgical Instruments for Rodent Catheterization

When preparing for survival microsurgery, there is a lot to consider. Surgical skills aside, being fully prepared using atraumatic and aseptic techniques, and having the right tools for the job, can make the difference between success and failure. Here is our guide on what the right tools are and their purpose.

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General Guidelines to Follow

Instruments should not cause undue trauma or add time to performing the surgical procedure. Scissors must be kept sharp to not crush tissues when skin incisions are made, for example. Surgical instruments need to feel right and should not be under, or oversized. Choose your instruments carefully because the bottom line is that they really are the surgeon's preference. But remember, buying high quality instruments is well worth the investment as they will last and stay sharper longer. Holding your instruments the right way also makes a big difference when you use them. To this point, forceps should be held using a pencil grip. Make sure you clean your instruments properly after use, protect the tip of sharp instruments, maintain them and sterilize them prior to surgery.

The Must Haves

Sometimes there just isn't a way around it. Here are the tools you simply cannot successfully complete a surgery without:

Scissors

Having different types of scissors is important since each scissors has a different purpose, it also supports a strict aseptic technique.

  • External Scissors: A dedicated pair of external scissors used for skin incisions and cutting external sutures minimizes the risk of infection supporting a strict aseptic technique. Some people prefer to use a scalpel rather than scissors for the skin incision. If you do use a scalpel, take caution to not damage underlying tissues.
  • Internal Scissors: A dedicated pair of internal scissors used for abdominal wall incision, sharp dissection of internal tissues and cutting internal sutures minimizes the risk of infection supporting a strict aseptic technique.
  • Vessel incision: A pair of delicate spring scissors used for incisions in vessels and fine membranes only. Cutting anything else with these scissors will damage  them. There are alternate ways to incise a vessel, such as using a needle, but the delicate spring scissors work very well.

Forceps

Multiple types of forceps are needed in order to carry out the different surgical steps and to support aseptic technique.

  • External use: A dedicated pair of forceps used to hold skin when making the incision. These forceps should cause minimal trauma to the skin and be used for that purpose only. Minimizes risks of infections supporting a strict aseptic technique.
  • Blunt dissection: These forceps are your go-to instruments to perform blunt dissection of tissues. The tips are blunt and serrated for a good grip while doing blunt dissection. They can also be used when suturing.
  • Vessel isolation: These fine blunt forceps will help isolate the vessel to be catheterized. The fact that they are blunt but also fine will be especially helpful while isolating vessels on small animals like mice. 
    Insertion of catheter in vessel: A sharp tip, angled forceps is most useful to hold the upper portion of the vessel incision site when insertion the catheter.

Hemostat

Hemostats are useful to make subcutaneous pockets and to use, if need be, to hold ends of a suture when applying tension.

Needle Holder

There are various types of needle holders to hold your needle for you while you suture. Choose one that you are comfortable with and easy for you to maneuver. Varieties include the one lock mechanism thumb release Kalt needle holder, the ratchet lock mechanism with finger rings such as the Mayo-Hegar and the ratchet lock mechanism with spring loaded palm-hand handles such as the Mathieu. Make sure to use the appropriate size needle holder; if you need to use micro sutures (7.0 and smaller) which come with fine needles, you need a micro needle holder such as Castroviejo micro needle holder. Do not use a micro needle holder on larger size suture (6.0 and larger) which comes with larger needles as you can damage the needle holder and it will not hold the needle properly. Micro sutures are usually used internally when doing purse sutures on vessels or intestines, for example. For skin or abdominal wall closure on rodents, which common size sutures are 5.0 or 6.0, you do not want to use a micro needle holder.

Nice to Haves

Retractor

Required for certain procedures, retractors are handy to have when retraction of  tissues is necessary for better visualization, as when performing a carotid artery catheterization. Never apply too much tension on tissues when retracting them.

Clamp

A clamp is used to temporarily occlude catheters when removing the syringe from its external end. A hemostat with covered jaws (instrument tip covers or silicone tubing can work great) can be used in lieu of a clamp.

Grooved Director

This odd instrument can be helpful to make subcutaneous tunnels and pockets. It is also helpful in guiding the catheter through the subcutaneous tunnel. If you do not have a grooved director, use a blunt tip instrument such as a hemostat to make the tunnels and pockets.

Suppliers

There are many surgical instruments and suppliers to choose from. To help you select your instruments and supplier, we have put together our own recommended surgical packs in a partnership with Fine Science Tools that include all the instruments listed above.

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The history of cutting blades

Surgeons today have access to elite power tools that their medieval and prehistoric ancestors couldn't begin to imagine.

Even as recently as the s, bladed surgical instruments could be considered rudimentary at best, and were mainly used for radical, traumatic procedures.

However, with the advent of electricity, surgeons' tools started to become motorized, and throughout the s great strides were made in the development of power tools that improved their accuracy, precision, and overall outcomes.

While much study has been made of the historical development of surgeons' tools, one crucial component has received far less attention: the blade.

The bone cutting blade has come a long way from the early days of flint and copper. The last few decades alone have seen great advances in blade technology. But while hospitals are keen to keep up with the latest gadgets, they often neglect to hold the arguably most critical component of these tools to the same standards.

In this article, we'll explore the history of bone cutting blades and highlight some recent advances that make it critical for surgeons to not only upgrade their power tools, but also the blades that power them.

Before even the most rudimentary blades were invented, basic procedures such as cutting umbilical cords, opening abscesses, and scarification still needed to be performed. Without any other alternative, prehistoric man used nature's own tools to do those jobs: fingernails, sharp leaves and stems, shells, sharpened bones, and animal teeth.[1]

The first signs of blades being used for cutting bones appeared around -BC. In this period, there are indications of flint knives being used to drill a hole in the skull, a procedure that was intended to release the 'demons' presumed to cause, among other things, headaches, depression, and epilepsy.[2]

Around BC, the first metallic blades were introduced along with the process of smelting copper ore.[3] Stronger iron and steel-facing blades came along with the developments of iron and steel working processes around BC and BC, respectively.[4]

As early as BC, blades were apparently strong enough to cut straight through bone, as evidenced by references to hand amputation in the code of Hammurabi from circa BC.[5]

In 400BC, Hippocrates mentioned the use of iron blades for scarification. This was the first indication of blades being used for proper surgical procedures.[6] From the 4th century BC onwards, ever newer and better blades would be developed and used for more procedures besides scarification.

In the second century A.D., the Romans were developing all kinds of bladed instruments for surgical procedures (Ancient Romans coined the term 'scalpel' from the Latin 'scallpellus'). Their cutting instruments used for surgery, such as knives, lances, and spears, were all made from bronze and iron.[7]

When the fall of the Roman Empire led into the Dark Ages, medical knowledge stagnated and many advances that had already been made were lost to time.[8]

It wasn't until the s, in the midst of the Renaissance, that physicians started to explore better procedures for cutting through bones.

Up until that point, untrained barber surgeons, so named because their profession also entailed cutting hair and pulling teeth,[9] would undertake limb amputations with tools like axes, saws, and heavy chisels.

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French physician Ambroise Pare (pictured below) rose from a barber surgeon to become Henri II's personal physician in the s. He began to develop amputation procedures that did a better job of controlling blood loss and ensuring good stump coverage.[10]

He is also known for creating scalpels with beautifully ornamented handles that were very popular during his time and for several hundred years afterwards.[11]

In , the first true flintlock mechanism was invented, which made firearms the weapon of choice in combat. As gun technology and the war wounds it inflicted changed, so too did the technology behind the surgical procedures, such as amputation, that were performed by physicians. [12]

In the s, surgeons used special curved knives to cut through skin and muscle at the amputation site, before using amputation saws to cut through the bone.[13]

With the development of anesthetic techniques in the s came the ability to perform quicker and less psychologically traumatic surgical procedures.[14] Makers of surgical instruments had also begun to develop all-metal tools, which were more antiseptic.[15] Amputations were still the most prevalent method of treating many gunshot injuries during the Civil War[16], but when it came to other bone problems, more delicate procedures were starting to be developed.

Anthony White, a surgeon in Westminster Hospital in London, performed the first successful hip arthroplasty in .[17]

In , Dr. Richard Butcher, an English surgeon, developed a frame saw for knee joint resection that had an adjustable rotating blade which could cut at any angle.[18] German surgeon Themistocles Gluck began development of total knee arthroplasty around .[19]

These techniques would, in the coming decades, begin to develop into the procedures surgeons practice today.

The s saw the development of many of the techniques and tools that modern surgeons know and practice. In , Morgan Parker invented the scalpel that is still used in modern operating rooms, a two-piece instrument consisting of blade and handle.[20]

The s saw the real development of the total knee replacement procedure. Frank Gunston designed an unhinged knee that helped make the procedure possible. Surgeons performed the first knee replacement surgery in , and improvements on the procedure and components continued through the s and s.[21]

It was in this climate of progress that Stryker grew and developed out of one doctor's desire to meet his patients' medical needs.

Its first Sagittal Saw was released in the s with the series blade. The Dual Cut blade for the Sagittal Saw, released near , introduced a unique and unprecedented tooth design known as offset tooth geometry. This change made these blades the most efficient across any blade series on the market and allowed surgeons to cut more accurately and quickly, creating less heat during the operation.

These blades became the cutting standard in the operating room until , when Stryker launched their Performance Series proprietary blade technology.

The patented teeth on the Performance blade feed bone chips into a chip clearance area at the top of the blade. This is a unique design that aims to reduce the amount of bone fragment buildup on the blade and bone fragement dispersal in the operating room.

Notice the chip clearance area on the right-hand side of Stryker's Performance Series blade, just below the teeth.

The Performance blade's proprietary technology is designed to maximise the performance of the Stryker Sagittal Saw.

But the history of Stryker's blade and saw technology doesn't stop there.

Stryker developed the Precision Saw as an upgrade to the Sagittal Saw. With the Precision Saw, Stryker sought to further improve upon the technology that had already made such a dramatic difference in the operating room.

Whereas the Sagittal Saw is compatible with the blade, the Dual Series blade, and Performance Series blade, the Precision Saw only uses the Precision Falcon Cartridge (pictured below). A dedicated handpiece allows the oscillating tip of the cartridge to function.

Unlike the blades in the Sagittal Saw, only the teeth at the top of the Precision cartridge move during operation. This unique tip allows surgeons to have even greater control over their cut.

The Precision cartridge aims to offer increased cutting speeds and reduced surgical noise, while the smaller blade surface area is designed to decrease the cutting temperature.

Or imagine you're a sprinter at the Olympics going to the starting line of the 100-meter dash in a pair of shoes developed 20 years ago. Sure, you might be a phenomenal athlete, but you're not taking full advantage of the technology and developments that have occurred in shoes over the last two decades.

If we expect Olympic athletes and high-performance cars to use only the latest technology, how much more should we expect it in a hospital operating room where lives are on the line?

Stryker blades were designed for Stryker power tools by engineers who understand how the right blade technology works with the right power tool technology to get the best performance.

Stryker's proprietary Performance Series blades and Precision oscillating tip saw are the best technology for their Sagittal Saw and the most advanced technology in bone cutting tools available today. 

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